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Image Search Results
Journal: The Journal of general virology
Article Title: Recombinant rotavirus expressing the glycosylated S1 protein of SARS-CoV-2.
doi: 10.1099/jgv.0.001899
Figure Lengend Snippet: Fig. 5. Localization of SARS CoV-2 S1 protein in rSA11/S1f-infected cells. MA104 cells were mock infected or infected with rSA11/S1f and, at 9 h p.i., fixed with ice cold methanol. Afterwards, the cells were incubated with rabbit S1/ABclonal antibody, mouse NSP2 or NSP4 antibody, followed by Alexa 488 anti-rabbit IgG (green) and Alexa 594 anti-mouse IgG (red) to determine the locations of SARS-CoV-2 S1 and rotavirus NSP2 and NSP4 proteins. Nuclei were detected by staining with DAPI. Cells were analysed with a Nikon Eclipse NiE microscope (100× oil immersion objective) and images were captured with a Hamamatsu Orca-Flash 2.8 sCMOS high resolution camera. Images include 20 µm size bars.
Article Snippet: Cells were analysed with a
Techniques: Infection, Incubation, Staining, Microscopy